Replication of the escherichia coli K12 chromosome.
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چکیده
منابع مشابه
Replication of the Escherichia coli chromosome.
Cell size and DNA concentration were measured in Escherichia coli K-12 ET64. This strain carries a dnaA(Ts) mutation that has been suppressed by the insertion of the F plasmid into the chrontosome. ET64 can grow in a balanced steady state of exponential growth at the restrictive temperature for its dnaA allele (39°C), in which chromosome replication is controlled by the F plasmid, and at the pe...
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An enzyme system that replicates plasmids bearing the origin of the Escherichia coli chromosomes (oriC) has the following physiologically relevant features. The system (i) depends completely on low levels of exogenously furnished supercoiled oriC plasmids, (ii) uses only those plasmids that contain the intact oriC region of about 245 base pairs, (iii) initiates replication within or near the or...
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E. coli cells initiating chromosome replication after release from amino-acid starvation were incubated in [(3)H]thymine of moderate specific activity, followed by incubation in [(3)H]thymine plus [(3)H]thymidine of very high specific activity. The grain tracks produced in autoradiographs of chromosomes were denser on both ends than in the middle. The autoradiographic patterns are, therefore, e...
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The region that includes the dnaB locus on the E. coli K12 chromosome was shown to be duplicated at high frequency in cell populations. The duplications were shown to be arranged in tandem and segregated at various frequencies. Segregation was dependent on the recA recombination system, but independent of recB,C. Though most of the data was obtained with dnaB::Tn10 insertion mutants, the duplic...
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At all levels of Life, systems evolve on the 'scales of equilibria'. At the level of bacteria, the individual cell must favor one of two opposing strategies and either take risks to grow or avoid risks to survive. It has been proposed in the Dualism hypothesis that the growth and survival strategies depend on non-equilibrium and equilibrium hyperstructures, respectively. It has been further pro...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1967
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.58.5.1911